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. 2015 Feb 20;290(16):10057–10070. doi: 10.1074/jbc.M114.624684

TABLE 2.

S. cerevisiae strains used in this study

Strain name Genotype
SF1 MATa ade2-1 can1-100 his3-11 leu2-3,112 trp1 ura3-1
SF1 Spt7-TAP MATa SPT7-TAP::URA3 ade2-1 can1-100 his3-11 leu2-3,112 trp1 ura3-1
BJ2168 MATa leu2 trp1 ura3-52 prb1-1122 pep4-3 prc1-407 gal2
BJ2168 Spt7-TAP MATa SPT7-TAP::URA3 leu2 trp1 ura3-52 prb1-1122 pep4-3 prc1-407 gal2
BJ2168 Spt7-TAP Δada2 MATa ada2::KanMX6 SPT7-TAP::URA3 leu2 trp1 ura3-52 prb1-1122 pep4-3 prc1-407 gal2
BJ2168 Spt7-TAP Δsgf73 MATa sgf73::KanMX6 SPT7-TAP::URA3 leu2 trp1 ura3-52 prb1-1122 pep4-3 prc1-407 gal2
BJ2168 Spt7-TAP Gcn5-GFP MATa SPT7-TAP::URA3 GCN5-GFP::KanMX6 leu2 trp1 ura3-52 prb1-1122 pep4-3 prc1-407 gal2
BJ2168 Spt7-TAP Ada2-GFP MATa SPT7-TAP::URA3 ADA2-GFP::KanMX6 leu2 trp1 ura3-52 prb1-1122 pep4-3 prc1-407 gal2
BJ2168 Spt7-TAP Sgf29-GFP MATa SPT7-TAP::URA3 SGF29-GFP::KanMX6 leu2 trp1 ura3-52 prb1-1122 pep4-3 prc1-407 gal2
BJ2168 Spt7-TAP Spt3-GFP MATa SPT7-TAP::URA3 SPT3-GFP::KanMX6 leu2 trp1 ura3-52 prb1-1122 pep4-3 prc1-407 gal2
BJ2168 Spt7-TAP Spt8-GFP MATa SPT7-TAP::URA3 SPT8-GFP::KanMX6 leu2 trp1 ura3-52 prb1-1122 pep4-3 prc1-407 gal2
BJ2168 Spt7-TAP Spt20-GFP MATa SPT7-TAP::URA3 SPT20-GFP::KanMX6 leu2 trp1 ura3-52 prb1-1122 pep4-3 prc1-407 gal2
BJ2168 Spt7-TAP Taf5-GFP MATa SPT7-TAP::URA3 TAF5-GFP::KanMX6 leu2 trp1 ura3-52 prb1-1122 pep4-3 prc1-407 gal2
BJ2168 Spt7-TAP Taf9-GFP MATa SPT7-TAP::URA3 TAF9-GFP::KanMX6 leu2 trp1 ura3-52 prb1-1122 pep4-3 prc1-407 gal2
BJ2168 Spt7-TAP Taf10-GFP MATa SPT&-TAP::URA3 TAF10-GFP::KanMX6 leu2 trp1 ura3-52 prb1-1122 pep4-3 prc1-407 gal2
BY4741 MATa his3Δ1 leu2Δ20 met15Δ0 ura3Δ0
BY4741 Sgf73-TAP MATa SGF73-TAP::URA3 his3Δ1 leu2Δ20 met15Δ0 ura3Δ0
BY4741 Sgf73-TAP Spt7(1-1180) MATa SGF73-TAP::URA3 spt7-1180::KanMX6 his3Δ1 leu2Δ20 met15Δ0 ura3Δ0