FIGURE 4.
The HC33.1-E2412–423 binding interface. A, close-up view of the interactions between region 1 of E2412–423 (yellow) and the VL (cyan) and VH (blue) domains of HC33.1. The side chains of contacting residues are shown in ball and stick representation with carbon atoms in yellow (E2412–423), cyan (VL), or green (VH); nitrogen atoms in blue; and oxygen atoms in red. Hydrogen bonds are drawn as dotted black lines. B, interactions between region 2 of E2412–423 and HC33.1, showing contacts made by the peptide β-turn. C, interactions between region 3 of E2412–423 and HC33.1. The side chain of Trp-420 binds in a β-barrel formed by VL strands F and G and VH strands C, C′, C″ F, and G (labeled).