TABLE 2.
Mutant | ΔΔG scorea |
---|---|
Q412A | 0.4 |
L413A | 2.4 |
I414A | 0.5 |
N415A | 0.1 |
T416A | 1.3 |
N417A | −0.1 |
G418A | −0.6 |
S419A | 0.9 |
W420A | 5.4 |
H421A | 1.9 |
I422A | 1.0 |
N423A | 0.0 |
L413I | 1.7 (1.9) |
N417T | 0 (0.1) |
S419N | 0.6 (0.4) |
a The Rosetta ΔΔG score was calculated with rotamer-based packing of the mutant residue, and for non-alanine mutants, backbone, and off-rotamer side chain, minimization of mutant and interface residues was also performed (in parentheses). Predicted substantially destabilizing mutants (ΔΔG scores >1.5) are shown in bold type.