OTP84 truncation shows differential effects on the editing of three cognate sites.
A–C show the response of editing ndhF C290, psbZ C50, and ndhB C1481, respectively. Truncation of the DYW domain (OTP84trcDYW) resulted in a large decrease in editing of the ndhF and psbZ sites, but the ndhB site was not significantly reduced. Three leaf samples were analyzed from three wild-type plants (WT). Three leaf samples were analyzed from each of three independent integration events for the OTP84trcDYW in the otp84-2 mutant background (trcDYW), and these samples are labeled 1, 2, and 3. Three leaf samples were analyzed from each of three independent integration events were analyzed for the OTP84trcPG in the otp84-2 mutant background (trcPG plant 1) or the otp84-3 background (trcPG plants 2* and 3*). Error bars show the S.D. of the three leaf samples from three independent plants in the case of WT and three leaf samples from a single plant in all other plants.