FIGURE 4.

CREF7-E554A does not restore editing in CREF7 knock-out plants. Wild type CREF7 complemented the knock-out phenotype when expressed in the CREF7 knock-out line; however, CREF7-E554A only increased editing of ndhB C1255 to about 10%. A single leaf sample was analyzed from three independent wild type (WT) and cref7-1 knock-out (KO) plants. A single leaf sample was analyzed from each of three independent integration events for these backgrounds and transgenes: wild type plants with CREF7 transgene (WT + CREF7); cref7 with CREF7-E554A (KO + CREF7-E554A); and wild type expressing CREF7-E554A (WT + CREF7-E554A).