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. 2015 Feb 25;290(16):10191–10199. doi: 10.1074/jbc.M114.612374

FIGURE 1.

FIGURE 1.

Retrotransposition of LINE-1 induces IFNβ expression. A, relative mRNA levels of indicated genes in the testes of 2-month-old Mov10l1 heterozygous or homozygous knock-out mice assessed by qPCR (levels in heterozygous mice taken as 1.0). Average from three independent experiments is shown as mean ± S.E. Here and thereafter: *, p < 0.05; **, p < 0.01; ***, p < 0.001. B, relative IFNβ mRNA levels (compared with Mock taken as 1.0) in mouse embryonic fibroblast cells transfected with LINE-1-expressing vectors that encode functional (EN+) or endonuclease-deficient (EN−) ORF2 protein. C, immunofluorescent analysis of IFNβ expression in NIH3T3 cells transfected with indicated RNAi oligos and indicated LINE-1 constructs (left panel; magnification bar: 10 μm). Right panel: quantification of percent of cells single or double positive for GFP and IFNβ proteins in total 25 fields randomly chosen from three independent experiments performed as described in left panel.