FIGURE 2.

LINE-1-induced IFNβ can up-regulate IFN-stimulated genes. A, relative mRNA levels of indicated IFN-stimulated genes in mouse embryonic fibroblasts transfected with indicated LINE-1 plasmids were assessed by qPCR (levels in EN⁻-transfected cells taken as 1.0). B, immunofluorescent analysis of IRF7 expression in NIH3T3 cells cultured in the presence of indicated antibodies (control IgG or neutralizing antibody against mouse IFNβ, 10 μg/ml for 30 h) and transfected with indicated LINE-1 constructs (left panel; magnification bar: 10 μm). Right panel: quantification of percent of cells single or double positive for GFP and IRF7 proteins in total 30 fields randomly chosen from three independent experiments performed as described in the left panel.