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. 2015 Mar 3;290(16):10256–10273. doi: 10.1074/jbc.M114.614099

FIGURE 5.

FIGURE 5.

Full MS spectra of permethylated O-glycans released from RPTPζ/phosphacan enriched from early postnatal mouse brains. O-Glycans were released from RPTPζ/phosphacan-enriched samples by reductive β-elimination. Following permethylation, O-glycans were subjected to aqueous-organic partition to separate sulfated glycans from non-sulfated glycans. Full MS spectra are presented for the organic phase in positive ion mode (non-sulfated glycans) (A), aqueous phase in positive ion mode (sulfated glycans) (B), and aqueous phase in negative ion mode (sulfated glycans) (C).