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Fbw7 promotes ubiquitylation of GATA2 but not GATA1. HEK293 cells were transfected with indicated expression plasmids and were then incubated with MG132 for 6 h. Total cell lysates were subjected to IP with the indicated antibodies under native (A) or denaturing conditions (B and C), followed by immunoblotting with the indicated antibodies (see “Experimental Procedures”).
