FIGURE 10.

EtOH exposure affects mouse fracture callus protein composition. A–H, histological analysis of binge EtOH treatment on mouse fracture callus composition. Fracture callus sections were stained with hematoxylin and esosin, and antibodies against OPN, TGF-β1, and α-SMA. The black bar indicates the length of 60 μm. I, active TGF-β1 protein in mouse fracture callus. The concentration of active TGF-β1 protein in the tibial fracture callus from saline- and EtOH-treated mice 3 and 7 days post-fracture was measured using ELISA (*, p < 0.01).