FIGURE 7.

Global transcription in MSC is not affected by EtOH exposure. A, ChIP showing binding of RNA pol II to the GATA4 promoter in MSC following treatment with OPN and EtOH. EtOH did not alter RNA pol II binding to GATA4 promoter and subsequent transcriptional activation. B, graphical representation of MZF1 binding to the MZF1 promoter. Fold-enrichment was calculated using nonspecific IgG binding as an internal control. C, expression of GATA4 mRNA in OPN- and EtOH-treated MSC. GATA4 mRNA levels were unaffected by exposure to EtOH. Data are presented as mean ± S.E. of three experiments. Teh gel is representative of three experiments.