Skip to main content
. 2015 Feb 24;290(16):9959–9973. doi: 10.1074/jbc.M114.616888

FIGURE 9.

FIGURE 9.

OPN, EtOH, and TGF-β1 regulate SOX-9 and collagen-1 mRNA expression. A, expression of SOX-9 and collagen-1 mRNA. MSC were exposed to OPN and EtOH for 24 h, and SOX-9 and collagen-1 mRNA levels were measured using qRT-PCR (*, p < 0.0001). B, effect of TGF-β1 on collagen-1 mRNA expression in MSC grown in osteogenic inducing cell culture medium. Application of exogenous TGF-β1 to MSC partially reversed EtOH inhibition of OPN-induced collagen-1 expression (*, p < 0.0001). C, effect of TGF-β1 on SOX-9 mRNA expression in MSC grown in chondrogenic inducing cell culture medium. Exogenous TGF-β1 treatment also partially reversed EtOH inhibition of OPN-induced SOX-9 mRNA expression (*, p < 0.0001). Data are presented as mean ± S.E. of three experiments.