FIGURE 6.

The survivin heteromer decreases after YM155 in YM155-sensitive KATOIII cells but not MKN45 cells. A, MKN45 cells were immunoprecipitated with anti-survivin or anti-cIAP1 or anti-rabbit IgG antibodies. Interaction of survivin and cIAP1 was determined with Western blot analysis using anti-survivin or anti-cIAP1 antibodies. B, KATOIII and MKN45 cells were treated with YM155 (20 nm) for 48 h. Cell lysates were run on a native gel. Various heteromers of survivin were determined with Western blot analysis using anti-survivin antibody. C, KATOIII and MKN45 cells were treated with YM155 (20 nm) for 48 h and then cell lysates were immunoprecipitated (IP) using an anti-survivin antibody. Determination of the binding of survivin to the monomer or dimer of cIAP1 was determined via native PAGE using anti-cIAP1.