Figure 1.

Analysis of C. albicans and enolase antigens in CSF by slot-blot. 20 μl CSF samples were diluted with 180 μl TBS and were blotted onto a nitrocellulose membrane, which was incubated with the rabbit antiserum against C. albicans or enolase or recombinant MBP-enolase (primary antibody) as indicated and afterwards incubated with a rabbit anti rat IgG (secondary antibody). Positive control: control + 200 ng yeast protein or purified MBP-enolase. Negative control: control - corresponds to TBS alone.