Figure 4.

Immunohistochemistry analysis of brain sections from the frontal cortex of an ALS patient and a control. Brain sections (frontal cortex) from ALS patient 11 and control 6 were observed with a confocal laser scanning microscope. Sections were obtained from fixed tissue and immunohistochemistry analyses were carried out. Double immunofluorescence assay using anti-tubulin and anti-C. glabrata antibodies was carried out as detailed in Materials and Methods. DAPI appears in blue and anti-C. glabrata is shown in green. Human tubulin appears in red. The different panels in the figure are indicated.