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. 2015 Feb 4;36(3):355–367. doi: 10.1093/carcin/bgv006

Fig. 4.

Fig. 4.

Curcumin suppresses EMT and cancer stemness in 5FUR colorectal cancer cell lines. (A) Western blot analysis of ZEB1 in parental and 5FUR cell lines. (B) Western blot analysis of polycomb repressive complexes subunits and H3K27me3 in 5FUR cell lines. (C) qRT-PCR analysis of miRNA expression in HCT116-5FUR and SW480-5FUR cell lines treated with curcumin and/or 5FU, normalized to RNU6B. (D) Images of 3D cultures stained with toluidine blue 1 and 3 days after treatment with curcumin and/or 5FU in parental and 5FUR HCT116 cells. Apoptotic and degraded cells were quantified (left).