Table 2.
Contents of hexose-phosphates, UDP-glucose, and Suc-6-P in leaves of different SPS knock-out genotypes compared with the Col-0 wild-type controlSamples were taken from 6-week-old plants in the light 5h into the photoperiod and immediately snap-frozen in liquid N2.
| Plant line | Glc-6-P | Glc-1-P | Fru-6-P | UDP-Glc | Suc-6-P |
|---|---|---|---|---|---|
| Metabolite content in nmol g–1 FW–1 | |||||
| Col-0 | 129.2±25.8 | 30.6±2.3 | 99.5±21.4 | 27.5±11.2 | 1.3±0.3 |
| spsa1 | 163.5±29.1 | 33.3±4.8 | 81.6±18.4 | 37.6±22.6 | 1.0±0.1 |
| spsc | 139.5±19.4 | 29.3±0.1 | 102.3±7.7 | 36.4±24.1 | 1.3±0.2 |
| spsa1/spsc | 411.7±26.1* | 62.7±1.8* | 209.9±23.8* | 77.3±17.1* | ND |
Values represent the mean (±SD) of six individual plants.
An asterisk indicate a significant difference at P<0.05 according to t-test. ND, not detectable.