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. 2014 Jul 18;65(18):5385–5400. doi: 10.1093/jxb/eru297

Fig. 4.

Fig. 4.

Phenotypic features of Arabidopsis AtPP2CF1oe and ABI1oe transgenic plants. (A) 32-d-old wild-type (WT), AtPP2CF1oe (#1 and #2), and ABI1oe plants. Scale bar: 10mm. (B) Expression of AtPP2CF1 (left) and ABI1 (right) transcripts in AtPP2CF1oe or ABI1oe plants. Total RNA from aerial parts of 2-week-old seedlings grown aseptically on MS medium with gellan gum was isolated and subjected to RT-qPCR. The expression ratios of AtPP2CF1 and ABI1 to UBC9 were calculated for each sample. Values represent the mean ± SD of three independent experiments. (C) Seed germination rate of WT, AtPP2CF1oe, and ABI1oe plants in the presence of the indicated ABA concentrations. Seed germination rate is expressed as the percentage of developed green and expanded cotyledons at 5 d after stratification. Values represent the mean ± SE of six independent experiments. Different letters above the bars indicate statistically significant differences between the lines in the presence of the indicated ABA concentrations, assessed by one-way ANOVA with Tukey-Kramer multiple comparison tests at P < 0.05. (D) Water loss from detached aerial parts of 3-week-old WT, AtPP2CF1oe, and ABI1oe plants. Water loss is expressed as the percentage of initial fresh weight. Values represent the mean ± SD of three independent experiments.