Fig. 3. Affinity chromatography of cell extracts with Arl5.

(A) Comparison of the spectral counts for proteins isolated from lysates prepared from Drosophila heads by affinity chromatography with GST tagged forms of GTP-locked or GDP-locked Arl5. Abundant GTP-specific interactors are labeled, ochre dots indicating GARP subunits. Full list of bound proteins is in supplementary material Table S1. (B) Comparison of the spectral counts for the top twenty proteins found exclusively with the GTP-form of Arl5 in the affinity purification shown in (A) versus the same from an affinity purification performed with Arl5 bound to liposomes. Proteins in both datasets are indicated. See supplementary material Table S2 for the full list of proteins from the liposome purification.