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. 2015 Mar 6;4(4):573–583. doi: 10.1242/bio.20149977

Fig. 4. Injection of Smad1-AVA (S1-AVA) and Smad2-P445H (S2-P445H) act additively to cause expansion of eye field.

Fig. 4.

Whole mount in situ hybridization for the eye field marker, rax (A–E) conducted on stage 15 embryos unilaterally injected with 125 pg of S1-AVA RNA, 3 ng of S2-P445H and 100 pg of β-gal. Area of rax expression was calculated by measuring the region within the dashed yellow lines on each side of the midline (white dotted line) as shown. Graph shows the ratio of the area of the injected side to the uninjected side. Red β-gal stain indicates injected side. Scale bar, 500 µm. Error bars  =  ±s.e.m.; **p<0.01; ***p<0.001.