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. 2015 Mar 6;4(4):573–583. doi: 10.1242/bio.20149977

Fig. 6. DM and SB43 treatment results in eye field expansion.

Fig. 6.

(A–E) In situ hybridization for the eye field marker, rax, in embryos treated with DMSO (A), DM (B,C) or DM+SB43 (D,E) from stage 9–15. Eye field expansion was determined by measuring the area of rax expression (dashed yellow line) normalized to the area of the dorsal face of the embryo (dashed white line), as shown in the graph. Error bars  =  ±s.e.m.; ***p<0.001. Dotted line on graph separates single treatment from dual treatment. Scale bar, 500 µm; n = number of embryos.