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. 2015 Mar 19;4(4):645–657. doi: 10.1016/j.stemcr.2015.02.009

Figure 2.

Figure 2

Role of miR-302/367 Cluster in Regulation of Cell Cycle and Apoptosis of hESCs

(A) Cell cycle analysis of hESCs-expressing control-KRAB or TALE1-KRAB by flow cytometry. Cells were dissected and stained by Vybrant Dyecycle according to manufacturer’s instructions. A representative graph of analyzing cell cycle processed with the FlowJo program was shown in the left, and analysis of cell cycle phase distribution was shown in the right. Data are represented as mean ± SD of three independent experiments (∗p < 0.05, ∗∗p < 0.01).

(B) Analysis of proliferating hESCs by EdU staining. hESCs expressing control-KRAB or TALE1-KRAB were cultured in 24-well plate overnight and then followed by the addition of EdU solution for 1 hr. Cells were dissected for EdU detection using the Click-iT detection kit. Data are represented as mean ± SD of three independent experiments (∗∗p < 0.01).

(C) Flow cytometric analysis of apoptotic hESCs. Control-KRAB- or TALE1-KRAB-expressing hESCs were stained with Annexin V-APC and then analyzed by flow cytometry (left). The percentage of Annexin V+ cells was determined (right). Data are represented as mean ± SD of three independent experiments (∗∗p < 0.01).