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. 2015 Mar 19;4(4):645–657. doi: 10.1016/j.stemcr.2015.02.009

Figure 6.

Figure 6

Butyrate Alleviates Apoptosis from hESC Induced by Knockdown of miR-302/367 Cluster

(A) qPCR analysis of pri-miR-302/367 transcripts in hESCs expressing control-KRAB- and TALE1-KRAB treated with or without butyrate (0.25, 0.5 mM) for 72 hr. Data are represented as mean ± SD of technical replicates (n = 3). See also Figure S5.

(B) qPCR analysis of BNIP3L/Nix transcripts in hESCs expressing control-KRAB- and TALE1-KRAB treated with or without butyrate (0.25, 0.5 mM) for 72 hr. Data are represented as mean ± SD of technical replicates (n = 3).

(C) Effect of butyrate on apoptosis induced by knockdown of miR-302/367 cluster. hESCs expressing control-KRAB or TALE1-KRAB-hESCs were treated with or without butyrate (0.25, 0.5 mM) for 72 hr and then assessed by flow cytometric analysis after staining with Annexin V-APC. Data are represented as mean ± SD of three independent experiments (∗∗p < 0.01).

(D) Effects of butyrate on growth of hESCs with knockdown of miR-302/367 cluster. hESCs stably expressing TALE1-KRAB were culture in medium with or without butyrate and percentage of GFP+ cells were analyzed by flow cytometry. Data are represented as mean ± SD of three independent experiments (∗∗p < 0.01).