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. 2015 Apr 16;35(2):e00188. doi: 10.1042/BSR20140171

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© 2015 The Author(s) This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC-BY) (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC-BY) (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

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(a) The A_2AR eluted from the Ni²⁺–NTA linked agarose as a single band in silver-stained fractions with 250 mM imidazole. (b) Western blot of the 250 mM imidazole fraction with an anti-histidine antibody.