Figure 5. Thermostability of A_2aR–SMALP, DDM-solubilized A_2aR and membrane-bound A_2aR prepared from HEK293T cells.

A_2aR–SMALP (●), DDM-solubilized A_2aR (▲) or membranes (○), prepared from HEK293T cells transfected with A_2aR, were incubated for 30 min at the stated temperatures, then chilled on ice, before specific binding of [³H]ZM241385 was determined as described in ‘Methods’. Data are expressed as specific binding relative to the 20°C data point (mean ± S.E.M. of three separate experiments performed in triplicate).