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. 2015 Mar 5;4(4):658–669. doi: 10.1016/j.stemcr.2015.02.003

Figure 2.

Figure 2

Impaired Development of Neural Progenitors in L3MBTL1-KD Pluripotent Stem Cells

(A) KD of L3MBTL1 impairs the ability to form neuronal progenitor cells and neurons. Nestin and Tuj1 expression were evaluated in EBs by IF. DAPI served as control. (Top) The first line shows multiple EBs at lesser magnification (4×); the second line shows a single EB at 10× magnification. (Bottom) The impairment in neural progenitor formation of the L3MBTL1-KD EBs. The graph on the right shows the percentage of EBs containing Tuj1+ cells. The data represent the mean ± SD of the three independent experiments. ∗p < 0.05 by Student’s t test.

(B) KD of L3MBTL1 enhances CD34 expression. (Top left) The first line shows few positive CD34+ EBs at low magnification (4×) in the controls; the second line shows a single EB at higher magnification (10×). (Bottom left) Increased CD34 staining in the L3MBTL1-KD EBs at 4× and 10× magnification is apparent. (Top right) Increased expression of CD34 marker (21%) in the L3MBTL1-KD cells compared to controls (3%) by FACS is shown. (Bottom right) The graph gives the percentage of EBs containing CD34+ cells. The data represent the mean ± SD of the three independent experiments. ∗p < 0.05 by Student’s t test. See also Figure S2.