TABLE II.
Serum Cytokine Levels in Pg/Ml
| TNF-a | IL-1b | IL-6 | IL-17 | IFN-g | G-CSF | IL-9 | IL-13 | |
|---|---|---|---|---|---|---|---|---|
| wt 6 months vs. APCMin/+ 6 months | ||||||||
| WT | 638 (135) | 225 (22) | 18 (6) | 45 (7) | 142 (12) | 81 (101) | 138 (40) | 108 (23) |
| APCmin/+ | 153 (359) | 314 (38) | 90 (16) | 52 (11) | 136 (45) | 229 (93) | 591 (77) | 12 (35) |
| p-value | ns | ns | 0.027 | ns | ns | ns | 0.00002 | ns |
| wt 6.months vs.RagAPCmin/+ 6 months | ||||||||
| WT | 638 (135) | 228 (22) | 18 (6) | 45 (7) | 142 (12) | 81 (101) | 138 (40) | 108 (23) |
| RagAPCmin/+ | 699 (56) | 32 (153) | 113 (17) | 66 (16) | 190 (58) | 238 (114) | 538 (74) | 128 (14) |
| p-value | ns | ns | 0.039 | 0.034 | ns | ns | 0.00002 | ns |
| Apcmin/+ 6 months vs.Rag APCmin/+ 6 months | ||||||||
| APCmin/+ | 1537 (359) | 314 (38) | 90 (16) | 52 (11) | 136 (45) | 229 (93) | 591 (77) | 120 (35) |
| RagAPCmin/+ | 699 (56) | 327 (153) | 113 (17) | 66 (16) | 190 (58) | 238 (114) | 538 (74) | 128 (14) |
| p-value | ns | ns | ns | ns | ns | ns | ns | ns |
| Rag Apcmin/+ 6 months untreated vs.Treg | ||||||||
| Untreated | 699 (56) | 327 (153) | 113 (17) | 66 (16) | 190 (58) | 238 (114) | 538 (74) | 128 (14) |
| Treg | 500 (114) | 225 (28) | 20 (3) | 46 (13) | 115 (7) | 60 (20) | 151 (74) | 89 (11) |
| p-value | 0.016 | ns | 0.043 | ns | 0.021 | 0.010 | 0.00032 | 0.002 |
| Apcmin/+ 3 months vs.APCmin/+ 6 months | ||||||||
| 3 months | 619 (55) | 228 (22) | 16 (3) | 44 (7) | 143 (11) | 57 (29) | 112 (17) | 112 (6) |
| 6 months | 1537 (359) | 314 (38) | 90 (16) | 52 (11) | 136 (45) | 229 (93) | 591 (77) | 120 (35) |
| p-value | ns | ns | 0.023 | ns | ns | 0.013 | 0.00006 | ns |
Regulatory T cells restore immune homeostasis. Serum levels of cytokines IL-6 and IL-9 were significantly increased in aged Min mice at high risk of intestinal polyposis and prostate cancer. Supplementation with wt TREG cells restored immune homeostasis, prevented cancer development and decreased serum cytokine levels of aged Rag2−/−Min mice to those of wt mice or young adult Min mice. Serum cytokine levels in pg/ml; assay used sera of n = 5 mice per group. Standard error is presented in parentheses.
Statistics calculated using 2-tailed Student’s t-test ns, not significant (p = 0.05).