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SDS-PAGE analysis of the recombinant BoSA1 protein expressed in E. coli DH5α. Lane M, protein ladder (kDa). The predicted rBoSA1 protein band expressed in cells is indicated. The figure includes five randomly selected clones of E. coli DH5α cells transformed with recombinant plasmid pGEX-4T3/BoSA1. Lanes E1, E2, E3, E4, and E5, E. coli DH5α cells treated with IPTG. Lanes C1, C2, C3, C4, and C5, uninduced control E. coli DH5α cells.
