FIG 3.

Western blot analysis of recombinant and native BoSA1 proteins. (A) Western blots showing the reactivity of anti-B. ovis antibodies with rBoSA1. The anti-B. ovis antibodies reacted with several components of rBoSA1 between ∼70 and ∼40 kDa but not with the GST protein. (B) Western blots showing the reactivity of anti-rBoSA1 mouse antibodies with native BoSA1 proteins in the erythrocytes and plasma of B. ovis-infected blood. Lane M, protein ladder; lane iRBC, infected erythrocyte lysate extracted with saponin; lane iP, infected plasma proteins precipitated with saturated ammonium sulfate; lane niRBC, noninfected erythrocyte lysate extracted with saponin; lane niP, noninfected plasma proteins precipitated with saturated ammonium sulfate. Comparisons with molecular size markers indicated estimated molecular masses consistent with the monomeric, dimeric, and tetrameric forms of native BoSA1.