TABLE 1.
Virological findings from patient specimens
| Specimen type | Result obtained usinga: |
|||
|---|---|---|---|---|
| HHV PCR | HEV PCR (CT value) | Respiratory virus multiplex PCRe | Viral culture | |
| Nasopharyngeal aspirate | Neg | ND | Pos for HEV/HRV | Posd |
| Blood (serum) | Negb | Pos (28)d | ND | Posd |
| Stool | ND | Pos (29)d | ND | ND |
| Cerebrospinal fluid | Neg | Pos (45) | ND | ND |
| Frozen lung tissue from autopsyc | ND | Pos (24)d | ND | ND |
Neg, negative; Pos, positive; ND, not done; HHV PCR, human herpesvirus PCR; HEV PCR, human enterovirus PCR (Cepheid); CT, cycle threshold. Tests for HHV 1 to 3 were conducted using LightMix kit HSV-1/2 (herpes simplex virus)/VZV (varicella-zoster virus) (TIB Molbiol). Tests for HHV 4 to 6 were conducted using Argene kits (bioMérieux). PCRs for HHV 1 to 6 were performed on a LightCycler 480 (Roche, Meylan, France).
Only HHV 4 to 6 were tested in serum.
Tested negative for Pneumocystis jirovecii.
Typed as HRV-C8 by sequencing.
The respiratory virus multiplex PCR assay used was the Respifinder SMART 22, which allows the detection of 18 viruses (rhinovirus/enterovirus, influenza A/B/A H1N1, adenovirus, metapneumovirus, respiratory syncytial viruses A and B, parainfluenza viruses 1 to 4, coronavirus NL63/OC43/229E/HKU1, and bocavirus) and 4 bacteria (Chlamydia pneumoniae, Mycoplasma pneumoniae, Legionella pneumophila, and Bordetella pertussis). This assay was performed on a LightCycler 480.