FIG 2.
Melting-curve graph for Mycobacterium avium subsp. silvaticum (MAS) identification by duplex real-time PCR. The graph displays the melting curves of reference strains Mycobacterium avium subsp. avium (MAA) ATCC 25291 and Mycobacterium avium subsp. silvaticum ATCC 49884 in the developed duplex HRM and M. avium subsp. silvaticum-specific real-time PCR assay. The M. avium subsp. silvaticum-specific MM amplification product has a Tm of 94.9°C, while the obtained HRM products had Tms of 91.15°C and 91.65°C for the M. avium subsp. silvaticum and M. avium subsp. avium genotypes, respectively.