TABLE 4.
Genes resulting from comparison of blood isolates with sputum isolatesa
| Gene | Annotation or putative gene function | Fold change |
|---|---|---|
| Metal ion transport or metabolism | ||
| BCAM2233 | Putative pyochelin biosynthetic protein PchC | 2.7 ↓ |
| BCAM0810 | Putative aromatic oxygenase | 2.5 ↑ |
| BCAM0811 | Putative aromatic oxygenase | 3.1 ↑ |
| Carbohydrate transport and metabolism | ||
| BCAL2793 | Major facilitator superfamily protein | 3.5 ↓ |
| BCAL2472 | Alpha, alpha-trehalose-phosphate synthase, otsA | 2.1 ↑ |
| BCAM0783 | Major facilitator superfamily protein | 3.4 ↑ |
| Cell envelope biogenesis, membrane proteins | ||
| BCAM0081 | Putative outer membrane protein-OmpW family | 2.4 ↓ |
| BCAL1391 | Cellulose biosynthesis protein | 2.1 ↓ |
| BCAL1395 | Cellulose synthase catalytic subunit, bscA | 2.9 ↓ |
| BCAL1396 | Cellulose synthase operon protein YhjU | 2.2 ↓ |
| BCAM0805 | Muconate cycloisomerase I 1, catB1 | 2.9 ↑ |
| BCAM1004 | GDP-mannose 4,6-dehydratase, gca | 2.6 ↑ |
| BCAM1008 | Glycosyltransferase | 2.9 ↑ |
| BCAM1010 | Putative UTP-glucose-1-phosphate uridylyltransferase, gtaB | 3.5 ↑ |
| Lipid transport and metabolism | ||
| BCAM0080 | AMP-binding domain-containing protein | 2.7 ↓ |
| BCAM1005 | Putative acyltransferase | 3.2 ↑ |
| Energy production | ||
| BCAL2795 | Aldehyde dehydrogenase family protein | 2.1 ↑ |
| BCAL3184 | Homogentisate 1,2-dioxygenase, hmgA | 3.2 ↑ |
| BCAL3187 | FADb-dependent oxidoreductase | 2.4 ↑ |
| Regulatory proteins | ||
| BCAL0534 | Two-component regulatory system, response regulator protein | 2.1 ↓ |
| BCAM2329 | GntR family regulatory protein | 2.0 ↓ |
| BCAL3205a | Putative phage-related regulator | 2.1 ↓ |
| BCAM1290 | RpiR-family transcriptional regulator | 2.2 ↓ |
| BCAM0835 | AraC family regulatory protein | 3.7 ↓ |
| BCAM0784 | LysR family regulatory protein | 2.0 ↑ |
| Quorum sensing | ||
| BCAM1870 | N-Acyl-homoserine lactone synthase CepI | 3.9 ↑ |
| BCAM1871 | Hypothetical protein | 4.5 ↑ |
| Secretion | ||
| BCAM2041 | Yop virulence translocation protein R | 4.2 ↑ |
| BCAM2042 | Lytic transglycosylase | 4.1 ↑ |
| BCAM2043_J_0 | Putative uncharacterized protein | 2.4 ↑ |
| BCAM2044 | Asparagine synthetase | 3.0 ↑ |
| BCAM2046 | Type III secretion protein SctT | 2.6 ↑ |
| BCAM2048 | Type III secretion apparatus H+-transporting two-sector ATPase | 4.9 ↑ |
| BCAM2049 | BcscL, type III secretion apparatus protein | 4.9 ↑ |
| BCAM2050 | BcscK, putative uncharacterized protein | 7.3 ↑ |
| BCAM2051 | Type III secretion apparatus lipoprotein | 3.8 ↑ |
| BCAM2052 | Uncharacterized protein | 3.4 ↑ |
| BCAM2053 | Uncharacterized protein | 6.9 ↑ |
| BCAM2055 | Type III secretion outer membrane pore | 4.0 ↑ |
| Exopolysaccharide | ||
| BCAM0859 | Capsular exopolysaccharide family | 2.8 ↑ |
| BCAM0860 | Glycosyl transferase | 4.7 ↑ |
| BCAM0861 | Glycosyltransferase-like protein | 3.1 ↑ |
| BCAM0863 | Glycosyltransferase | 3.0 ↑ |
| BCAM0864 | Glycosyltransferase | 2.0 ↑ |
| BCAM0856_J_0 | Sugar transferase | 2.1 ↑ |
| BCAM0856_J_1 | Sugar transferase | 4.9 ↑ |
| Motility and adherence | ||
| BCAL1677 | Type 1 fimbrial protein | 3.4 ↓ |
| Biofilm | ||
| BCAM0184 | Fucose-binding lectin II, photopexin A | 3.3 ↑ |
| Other proteins | ||
| BCAL2025 | Hypothetical protein | 2.0 ↓ |
| BCAL2793 | Major facilitator superfamily protein | 3.5 ↓ |
| BCAL3207 | Hypothetical protein | 2.5 ↓ |
| BCAM0414 | Hypothetical protein | 2.3 ↓ |
| BCAM2685 | Hypothetical protein | 2.6 ↓ |
| BCAL3006 | Cold shock-like protein | 2.2 ↑ |
| BCAL3186 | Hypothetical protein | 2.4 ↑ |
a
Irrespective of culture medium used, defined in Table 3.
b
FAD, flavin adenine dinucleotide.