TABLE 3.
Detection of HIVDR mutations in corresponding plasma samples by ARMS-PCR compared with sequence analysisa
| Mutation | No. of samples |
Sensitivity (%) | Specificity (%) | PPV (%) | NPV (%) | |||
|---|---|---|---|---|---|---|---|---|
| POS ARMS-PCR | POS sequencing | False-POS ARMS-PCR | False-NEG ARMS-PCR | |||||
| M184V | 35 | 32 | 4 | 1 | 96.8 | 90.6 | 88.5 | 97.5 |
| T215Y/F | 15 | 14 | 3 | 2 | 85.7 | 95 | 80 | 96.6 |
| K103N | 21 | 23 | 0 | 2 | 91.3 | 100 | 100 | 96.2 |
| Y181C | 9 | 10 | 2 | 3 | 70 | 96.9 | 77.7 | 95.4 |
a
POS, positive; NEG, negative; PPV, positive predictive value; NPV; negative predictive value. Sensitivity, specificity, PPV, and NPV of ARMS-PCR were calculated using sequencing as the gold standard.