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. 2015 Mar 27;194(9):4498–4506. doi: 10.4049/jimmunol.1401399

FIGURE 5.

FIGURE 5.

The expression of TNF-α, but not of IL-1β, is dependent on IRE1α endonuclease activity. RAW 264.7 cells were treated with TM (10 μg/ml) for 1 or 3 h in the absence or presence of STF083010. (A) Activation of XBP-1 and CHOP mRNA expression were determined by RT-PCR. GAPDH served as the standard. The expression of (B) IL-1β and TNF-α, (C) XBP-1 splicing, and (D) CHOP mRNA was determined by quantitative real-time PCR. GAPDH served as the standard. Data represent means ± SD of three independent determinations. **p < 0.01, ***p < 0.001.