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. Author manuscript; available in PMC: 2015 Oct 16.
Published in final edited form as: Nat Commun. 2015 Apr 16;6:6823. doi: 10.1038/ncomms7823

Figure 3. Activation of CMA protects against double strand DNA damage.

Figure 3

a. Representative immunoblot for LAMP-2A in mouse fibroblasts control (Ctr) or knock-down for LAMP-2A (L2A(−)) after 24h of treatment with etoposide. b. L2A mRNA levels cells treated with etoposide for 24h and 24h after treatment. Paraquat (PQ) is shown as positive control, n=3 wells from 3 independent experiment. c,b. Immunofluorescence for γH2AX in Ctr cells transfected with GFP or GFP-hL2A and exposed to etoposide for 24h. n = 3 independent experiments where the number of total cells counted per experimental condition were more than 100 (>5 fields, average 20 cells/field) (c) and representative images in single and merged channels images (d). Dashed lines: Cell profiles. e–g. Viability (e) and γH2AX levels (f,g) of cells pretreated or not with the CMA activator AR7 after 24h of etoposide treatment (damage) or 24h after removing the drug (recovery), n=3 independent experiments. All values are mean+s.e.m. (unpaired two-tailed t-test). *P<0.05 or **P<0.005. Scale bar: 10μm. Full gels are shown in Supplementary Fig. 8.