Figure 1. 1,25(OH)2D3 upregulates VDR expression in NSCs.
NSCs at the 5th passage were cultured in the presence or absence of 1,25(OH)2D3 (10−12–10−6 M) for 5 days, and VDR mRNA expression was determined with quantitative RT-PCR. Experiments were performed in triplicate using VDR and GAPDH primers. Data represent the mean ± SEM of triplicate samples analyzed. **P < 0.01, and ***P < 0.001, compared with cultures without 1,25(OH)2D3. One representative of three experiments is shown.