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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Exp Mol Pathol. 2015 Feb 11;98(2):240–245. doi: 10.1016/j.yexmp.2015.02.004

Figure 2. 1,25(OH)2D3 enhances NSC proliferation.

Figure 2

NSCs at the 5th passage were cultured in the presence or absence of 1,25(OH)2D3. (A) Phase-contrast images of NSCs were taken at days 9 and 14 after starting culture with 1,25(OH)2D3 at 10−6 M (scale bar = 100 µm). (B) At days 1, 3, 5, 9, and 14 after starting culture with 1,25(OH)2D3 at 10−6 M, neurospheres in each well were dissociated into single cells and cell numbers per well were counted by hemocytometer. At least five wells were assessed at each time point. (C) Analysis of NSC growth by BrdU incorporation assay. At day 14 of culture in the presence or absence of 1,25(OH)2D3(10−12–10−6 M), culture medium was supplemented with BrdU for 4–6 hrs and incorporation of BrdU was analyzed by ELISA (absorbance wavelength). Data represent the mean ± SEM of triplicate samples. *P < 0.05, **P < 0.01, ***P < 0.001, comparison between 1,25(OH)2D3-treated and untreated cells. One representative of three experiments is shown.