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. Author manuscript; available in PMC: 2015 Oct 16.
Published in final edited form as: Nat Commun. 2015 Apr 16;6:6746. doi: 10.1038/ncomms7746

Figure 4. Mus81 depletion does not affect replication initiation patterns.

Figure 4

Locations of replication initiation events were determined by massively parallel sequencing of nascent strands from Mus81-proficient HCT116 cells and from HCT116 cells that lacked Mus81 (designated Mus81 (−/−). (A–C) Initiation profiles were visualized using the Broad Institute Integrated Genome Browser. Representative screen shots of replication-initiation profiles from three genomic loci are shown at different zoom levels. These loci include (A) 72 kb from the hemoglobin, beta (HBB) locus on Chromosome 11, (B) 195 kb from the CCCTC-binding factor (CTCF) locus on Chromosome 16, and (C) 1857 kb from Chromosome 14. It should be noted that the genome browser images are presented on a relative scale; therefore, the sizes of the peaks (calibrated per total numbers of aligned reads in each sample) indicate that initiation events preferentially occur at particular genomic locations but do not directly correspond to absolute initiation frequencies. (D) Locations of replication initiation events were determined by massively parallel sequencing of nascent strands from: 1) HCT116 cells (two separately sequenced samples designated HCT116 (1) and HCT116 (2)), 2) HCT116 cells that lacked Mus81 (designated Mus81 (−/−)), and 3) MCF7 cells. Locations of replication initiation sites were calculated using two bin sizes: 100 and 1000 bp. Shown are Pearson’s correlation coefficients concerning the location of replication initiation sites for comparisons between the HCT116 (1) sample and indicated samples.