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. Author manuscript; available in PMC: 2015 Oct 16.
Published in final edited form as: Nat Commun. 2015 Apr 16;6:6746. doi: 10.1038/ncomms7746

Figure 8. Effects of depletion of XPF on replication fork progression in Mus81−/− cells.

Figure 8

HCT116 and Mus81−/− cells were transfected with siRNA directed against XPF or control siRNA. (A) Western-blot analysis revealed an efficient knockdown of XPF protein via siRNA treatment. (B-C) Cells were sequentially pulsed with IdU and CldU for 20 minutes, respectively before harvesting for DNA combing. Scatter plot was used to show the distribution of replication fork speed (B) and interorigin distance (C). Median with interquartile range was shown for the scatter plot. Mann Whitney test was used for the statistical analysis. A few extreme numbers (>4.5 kb/min for fork speed, >400 kb for origin distance) were not shown for a better resolution of the scatter plot. Statistical analyses were shown in Supplementary Table 4.