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. Author manuscript; available in PMC: 2016 Feb 27.
Published in final edited form as: J Mol Biol. 2014 Dec 24;427(4):792–806. doi: 10.1016/j.jmb.2014.12.010

Fig. 2.

Fig. 2

In vitro RNA synthesis from wild type and mutant gal DNA templates in the absence (−) and presence (+) of CCC. DNA templates with P1 numbering system from +1 to −25 and P2 from +6 to −20 are labeled as shown in the Fig. 1 The mutation above each lane is indicated: (a) +1 to −2, (b) −3 to −5, (c) −6 to −8 and (d) −9 to −11 represent P1, while (a) +6 to +4, (b) +3 to +1, (c) −1 to −3 and (d) −4 to −6 represent P2, P1, P2, P2*, and P3 represent gal promoters. RNAI transcript is used as an internal control.