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. Author manuscript; available in PMC: 2016 Mar 5.
Published in final edited form as: Cell Stem Cell. 2015 Mar 5;16(3):323–337. doi: 10.1016/j.stem.2015.01.015

Figure 4. Variability in Transgene Stoichiometry between Reprogramming Systems and within Each System.

Figure 4

(A) Percentage of cells with positive expression for each of the four transgenes over course of reprogramming. Percentages are based on the gates shown in Figures S4A and S4B.

(B) Percentage of cells that fall within each of 16 possible binary combinations of transgene expression over the course of reprogramming. Percentages are based on the gates from Figures S4A and S4B. Each binary combination is indicated by the key on the right, where black boxes denote positive expression above background.

(C) Cell-cycle gating strategy for assignment to G0, G1, S, G2, or M phase using IdU incorporation and the markers Ki67, phospho-Histone H3, and Cyclin B1.

(D) Percentage of cells in each cell-cycle phase for selected c-Mychigh and c-Myclow populations of the time course of Dox induction.

See also Figure S4.