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. Author manuscript; available in PMC: 2016 Mar 5.
Published in final edited form as: Cell Stem Cell. 2015 Mar 5;16(3):323–337. doi: 10.1016/j.stem.2015.01.015

Figure 6. End-Stage Trajectories of Cellular Reprogramming: MEF-like, ESC-like, and Lin28high.

Figure 6

(A) Time-resolved FLOW-MAP plots for Oct4-GFP primary MEFs, colored by protein expression level of the indicated markers. The end-stage trajectories are indicated by black arrows.

(B) Overview FLOW-MAP plot of the late stage reprogramming Oct4-GFP primary MEFs. Day 16 Dox release and the MET are indicated by dashed lines.

(C–E) Immunofluorescence microscopy of day 24 primary reprogramming MEFs stained with anti-Lin28 antibody (Alexafluor-488 secondary antibody), anti-Nanog antibody (Alexafluor-594 secondary antibody), and Hoechst dye. Representative images of a Lin28highNanoghigh colony with mESC-like morphology (C), a Lin28high Nanoglow colony with epithelial cobblestone morphology (D), and a Lin28highNanoglow colony with fibroblast-like morphology adjacent to a Lin28high Nanoghigh colony with mESC-like morphology (E). (F and G) Lin28high and Nanoghigh branches of the Oct4-GFP primary MEF time-resolved FLOW-MAP, colored by markers of pluripotency (F) and cell signaling (G).

See also Figure S6.