1	1. Gentamicin treatment [0.004% (4.32 mM)] of control and experimental fish for 24 hr at 28 °C using an incubator.
2	2. Wash out of gentamicin to initiate regeneration of hair cells. Return fish to the 28 °C incubator for investigator selected time periods between 8-16 hr.
2	3. Vital dye stain [0.08% 4-4-diethylaminostyryl-N-methylpyridinium iodide (4-Di-2-Asp)] control and experimental fish for 1 hr at room temperature and then wash out stain with fish water for fluorescent imaging.
1 or 2	4. If necessary due to nonsignificant results from analysis of neuromasts, repeat Protocols 1-3 with a separate group of control and experimental fish, but then obtain a skin preparation for confocal analysis of individual hair cells.