Reagent	Concentration in 5 μl	Volume	Volume
(1 rxn)	(96 rxns)
Water (HPLC grade)	NA	0.8 μl	92.2 μl
10x PCR Buffer with 20 mM MgCl2	1x (2 mM MgCl2)	0.5 μl	57.6 μl
MgCl2 (25 mM) **	2 mM	0.4 μl	46.1 μl
dNTP mix (25 mM each) ***	500 μM	0.1 μl	11.5 μl
Primer mix (500 nM each)	100 nM	1.0 μl	115.2 μl
PCR Enzyme (5 U/μl)	1.0 U/rxn	0.2 μl	23.0 μl
Total Volume:		3.0 μl	345.6 μl