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. 2015 Feb 28;(96):52385. doi: 10.3791/52385
Reagent Concentration in 5 μl Volume Volume
(1 rxn) (96 rxns)
Water (HPLC grade) NA 0.8 μl 92.2 μl
10x PCR Buffer with 20 mM MgCl2 1x (2 mM MgCl2) 0.5 μl 57.6 μl
MgCl2 (25 mM) ** 2 mM 0.4 μl 46.1 μl
dNTP mix (25 mM each) *** 500 μM 0.1 μl 11.5 μl
Primer mix (500 nM each) 100 nM 1.0 μl 115.2 μl
PCR Enzyme (5 U/μl) 1.0 U/rxn 0.2 μl 23.0 μl
Total Volume: 3.0 μl 345.6 μl