Reagent | Concentration in 5 μl | Volume | Volume |
(1 rxn) | (96 rxns) | ||
Water (HPLC grade) | NA | 0.8 μl | 92.2 μl |
10x PCR Buffer with 20 mM MgCl2 | 1x (2 mM MgCl2) | 0.5 μl | 57.6 μl |
MgCl2 (25 mM) ** | 2 mM | 0.4 μl | 46.1 μl |
dNTP mix (25 mM each) *** | 500 μM | 0.1 μl | 11.5 μl |
Primer mix (500 nM each) | 100 nM | 1.0 μl | 115.2 μl |
PCR Enzyme (5 U/μl) | 1.0 U/rxn | 0.2 μl | 23.0 μl |
Total Volume: | 3.0 μl | 345.6 μl |