| Reagent | Volume (1 rxn) | Volume (96 rxn) |
| Water (HPLC grade) | 1.53 μl | 176.3 μl |
| SAP Buffer (10x) | 0.17 μl | 19.6 μl |
| SAP enzyme (1.7 U/μl) | 0.30 μl | 34.6 μl |
| Total Volume | 2.00 μl | 230.4 μl |
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