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. 2014 Dec 11;7(2):143–153. doi: 10.1093/jmcb/mju047

Figure 6.

Figure 6

p38 signal transduction partially mediates MV-induced cancer cell invasion. (A) MMP mRNA expression in MCF-7 cells stimulated for 24 h with MV (25 µg/ml) (qRT–PCR, mean ± SD, n = 3). (B) Zymography for MMP-9 in tumor and benign cells (C), as well as their respective MV (SK, SK-BR-3; MΦ, human macrophages; P, platelets). (C) Western blots showing the phosphorylation of p38 and c-jun in MCF-7 cells stimulated with T-MV for the indicated time periods. (D) Analysis of p38 phosphorylation in MCF-7 cells incubated for 1 h with T-MV from EMMPRIN knockdown (shEMP) or non-sense control (ns) cells. (E) Microinvasion assay of MCF-7 cells pretreated with the p38 inhibitor SB-203580 (SB) for 2 h followed by stimulation with T-MV (1 µg/ml) (mean ± SD, n = 3, *P < 0.001). (F) Western blots showing p38 phosphorylation in MCF-7 cells pretreated with two EMMPRIN blocking peptides P-N160 and P-N268, respectively, for 2 h prior to stimulation with T-MV.