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Effects of several siRNA sequences and TNF treatment on the fraction of cells positive for VCAM1 and SELE. Each bar represents the mean of three independent experiments, error bars show standard deviations. (A) Effects of four different sequences (S1–S4) siRNA targeting SELE: S1: sense: (GGU UGA AUG CAC CAC UCA A)dTdT antisense: (UUG AGU GGU GCA UUC AAC C)dTdG S2: sense: (UGG UAG AAU UGG AGA GUA A) dTdT antisense: (UUA CUC UCC AAU UCU ACC A) dTdG S3: sense: (CAG UGU GGU UUG UGU UUG A)dTdT antisense: (UCA AAC ACA AAC CAC ACU G) dGdT S4: sense: (CGG AAG CUA UGA CUU AUG A)dTdT antisense: (UCA UAA GUC AUA GCU UCC G)dTdG (B) Effects of five different sequences (S1–S5) siRNA targeting VCAM1: S1: sense: (GGA GGA UAC GGA UAU GAA A)dTdT antisense: (UUU CAU AUC CGU AUC CUC C)dAdA S2: sense: (GAG CUA AAU UAC ACA UUG A)dTdT antisense: (UCA AUG UGU AAU UUA GCU C)dGdG S3: sense: (CAU CUA CGC UGA CAA UGA A)dTdT antisense: (UUC AUU GCU AGC GUA GAU G)dTdG S4: sense: (CUC UAU AUU UAG AUU GUU A)dTdT antisense: (UAA CAA UCU AAA UAU AGA G)dTdG S5: sense: (AAU GCA ACU CUC ACC UUA A)dTdT antisense: (UUA AGG UGA GAG UUG CAU U)dTdT