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6.

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Co-localization of Cav-3 and FAK / DDR-2 / vWF / c-Kit in Cav-1+/+ and Cav-1−/− mouse LVM. In Cav-1+/+ mouse some FB are Cav-3- (green) and FAK-positive (red) and the two proteins are partially co-localized (yellow) (large arrows), besides some FB are Cav-3-positive but FAK-negative (small arrows) (A–C). Some Cav-3-positive FB (red) are DDR-2-positive (green) and the two proteins are partially co-localized in FB tails (arrows) (E–G). vWF-positive CEC (arrows) do not have Cav-3 (I–K). ICLC are both Cav-3- (green) and c-Kit-positive (red) and the two proteins are partially co-localized (yellow) (M–O). In Cav-1−/− mouse Cav-3 appeared at CM plasma membranes, Z-lines and FB (arrows), but FAK and c-Kit are completely absent (D and P). DDR-2 and vWF shows at FB and CEC, respectively (H and I). Scale bar is 10 μm for a–d and i–p, and 7 μm for e–g.