1.
Antibody | Host | Dilution | Cat. No. | Source |
---|---|---|---|---|
Primary antibodies | ||||
MMP-2 | Mouse | 1:400 | MAB3308 | Chemicon International |
Cav-1 | Mouse | 1:50 | 610406 | BD transduction |
Cav-2 | Rabbit | 1:400 | ab2911 | Abcam |
Cav-3 | Mouse | 1:200 | 610420 | BD transduction |
Cav-3 | Rabbit | 1:200 | ab2912 | Abcam |
FAK | Mouse | 1:100 | MAB1144 | Chemicon International |
DDR2 | Rabbit | 1:10 | ab5520 | Abcam |
vWF | Rabbit | 1:200 | AB7356 | Chemicon International |
c-Kit | Mouse | 1:20 | 32–9000 | Invitrogen |
Secondary or tertiary antibodies | ||||
Fab fragment rabbit antimouse IgG | 325–007-003 | Jackson ImmunoResearch | ||
Cy3-donkey antimouse IgG | 715–165-151 | Jackson ImmunoResearch | ||
Alexa488-donkey antirabbit IgG | A-21206 | Invitrogen | ||
Sera | ||||
Mouse IgG blocking reagent | BMK-2202 | Vector | ||
Normal donkey serum | 566460 | Calbiochem |
MMP-2, matrix metalloprotenase-2; Cav-1, caveolin-1; Cav-2, caveolin-2; Cav-3, caveolin-3; FAK, focal adhesion kinase;
DDR2, discoidin domain receptor-2; vWF, von Willebrand factor; c-Kit, receptor tyrosine kinase